D6064
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D6064
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DNA Arrays: Methods and Protocols
| ISBN-10: | 0-89603-822-X |
| ISBN-13: | 978-0-89603-822-6 |
Description
| General description | Microarray technology is used in research of gene expression, mutation analysis, proteomics and gene sequencing. Protocols provide instruction in designing and constructing DNA arrays as well as hybridizing them with biological samples for analysis. Details include how to attach or print arrays on various matrices, biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies and data collection and analysis. Other topics are genotyping, sequencing by hybridization, antisense reagents, gene expression analysis, and business aspects of biochip technology. |
Properties
| publication info | J.B. Rampal, ed., Humana Press, 2001, 264 pp., hard cover |
Table Of Contents
| Table of Contents | DNA Microarrays: History and Overview Gel-Immobilized Microarrays of Nucleic Acids and Proteins: Production and Application for Macromolecular Research Sequencing by Hybridization Arrays Ethical Ramifications of Genetic Analysis Using DNA Arrays Photolithographic Synthesis of High-Density Oligonucleotide Arrays Automated Genotyping Using the DNA MassArrayTM Technolog Ink-Jet–Deposited Microspot Arrays of DNA and Other Bioactive Molecules Printing DNA Microarrays Using the Biomek 2000 Laboratory Automation Workstation Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays DNA Sequencing by Hybridization with Arrays of Samples or Probes, Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents Low-Resolution Typing of HLA-DQA1 Using DNA Microarray Gene Expression Analysis on Medium-Density Oligonucleotide Arrays Use of Bioinformatics in Arrays Confocal Scanning of Genetic Microarrays Business Aspects of Biochip Technologies. Index. |
